rPhl p 5 (Phleum pratense 5.0101)

rPhl p 5 (Phleum pratense 5.0101)
Regulatory status
RUO
Format
Purified Azide Free
Application
Variant
0.1 mg
10-P175-C100
In stock
242.00 USD
Variant
0.1 mg
10-P175-C100
In stock
242.00 USD
Product details
Description
Images
SDS download
Application
Description
Recombinant protein rPhl p 5 is expressed in Escherichia coli. DNA sequence encoding 300 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 30 kDa.
Contents
Recombinant protein rPhl p 5 - 1 vial (0.1 mg), lyophilized.
Reconstitute with sterile deionized water with or without azide.
Preparation
Purified by sequential steps of ion exchange and affinity chromatography. Lyophilized from a 0.2 μm filtered solution in Storage buffer (100 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 8.0).
Storage and handling
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
UniProt ID Q40960
10-P175_ELISA
ELISA (enzyme-linked immunosorbent assay) test was designed to prove the bond between the coated target recombinant allergen rPhl p 5 and allergen-specific human plasma IgG4 antibodies of Phleum pratense positive donor. A measurable signal was subsequently generated by the addition of biotin labeled anti-human IgG4 (detection) antibody, Streptavidin-HRP and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated rPhl p 5.
10-P175_čistota
Recombinant allergen rPhl p 5 purity verification. 5 µg of rPhl p 5 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.
10-P175_FC_Profil Značení
Flow cytometry dot-plot staining pattern of rPhl p 5 recombinant allergen stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using anti-human CD63 (MEM-259) FITC and anti-human CD203c (NP4D6) PE antibodies of BasoFlowEx kit.
10-P175_WB_Ponceau
Reactivity of rPhl p 5 with sIgE antibodies in plasma of a patient with confirmed presence (routine clinical test) of specific IgE antibodies to Timothy grass pollen allergen extract.

Western blotting analysis was performed on recombinant allergen rPhl p 5 mixed and heated (100°C, 5 min) with reducing SDS loading buffer. Sample was resolved using Tris-tricine gel electrophoresis. Nitrocellulose membrane blot was stained with Ponceau S to verify protein transfer. After thorough washing and blocking steps, the membrane was incubated with plasma of an allergic patient (1:10). Captured rPhl p 5 sIgE antibodies were detected by biotinylated anti-human IgE secondary antibody (2 μg/ml) followed by IRDye 800CW Streptavidin (1:5000).
Variant
0.1 mg
10-P175-C100
In stock
242.00 USD
Variant
0.1 mg
10-P175-C100
In stock
242.00 USD

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