Anti-Notch1 PE

Anti-Notch1 PE
Regulatory status
RUO
Antigen
Notch1
Clone
mN1A
Format
PE
Reactivity
Mouse, Human
Application
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-651-C100
In stock
198.00 USD

0.025 mg
1P-651-C025
Delivery 1 week
99.00 USD
Variant
0.1 mg
1P-651-C100
In stock
198.00 USD

0.025 mg
1P-651-C025
Delivery 1 week
99.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.
Application
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Reactivity
Mouse, Human
Negative species
Rat
Immunogen
GST fusion protein containing cdc10-NCR region of mouse Notch1
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
AOS5, TAN1, hN1, AOVD1
Antigen description
Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged 1 (CD339), and Jagged 2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus.
Entrez Gene ID 4851
UniProt ID P46531
1P-651_FC_Dot-plot
Flow cytometry multicolor surface staining pattern of human PHA stimulated CD3 positive lymphocytes using anti-human CD25 (MEM-181) APC antibody (10 μl reagent / 100 μl of sample) and intracellular staining of human lymphocytes using anti-Notch1 (mN1A) PE antibody (concentration in sample 3 μg/ml).
1P-651_FC_Histogram
Separation of CD3 positive CD25 positive cells stained using anti-Notch1 (mN1A) PE antibody (concentration in sample 3 μg/ml, red-filled) from CD3 positive CD25 positive cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 3 μg/ml, same as Notch1 PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of PHA stimulated human peripheral whole blood .
1P-651_FC_Profil Značení_2
Flow cytometry intracellular staining patterns of PHA stimulated human peripheral whole blood stained using anti-Notch1 (mN1A) PE antibody (concentration in sample 3 μg/ml, left) or mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 3 μg/ml, same as Notch1 PE concentration, right).

Product specific references:

Khwaja SS, Liu H, Tong C, Jin F, Pear WS, van Deursen J, Bram RJ: HIV-1 Rev-binding protein accelerates cellular uptake of iron to drive Notch-induced T cell leukemogenesis in mice. J Clin Invest. 2010 Jul 1;120(7):2537-48.
PubMed
Sun H, Li L, Vercherat C, Gulbagci NT, Acharjee S, Li J, Chung TK, Thin TH, Taneja R: Stra13 regulates satellite cell activation by antagonizing Notch signaling. J Cell Biol. 2007 May 21;177(4):647-57.
PubMed
Variant
0.1 mg
1P-651-C100
In stock
198.00 USD

0.025 mg
1P-651-C025
Delivery 1 week
99.00 USD
Variant
0.1 mg
1P-651-C100
In stock
198.00 USD

0.025 mg
1P-651-C025
Delivery 1 week
99.00 USD

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