Anti-N-sulfated heparan sulfate PE

Anti-N-sulfated heparan sulfate PE
Regulatory status
RUO
Antigen
N-sulfated heparan sulfate
Clone
HepSS-1
Format
PE
Reactivity
Species independent
Application
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-821-C100
In stock
264.00 USD
Variant
0.1 mg
1P-821-C100
In stock
264.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgM
Specificity
The mouse monoclonal antibody HepSS-1 (also known as HepSS1) recognizes N-sulfated heparan sulfate (extracellular antigen) present in many species.
Application
Application details
Flow cytometry: Recommended dilution: 2-5 μg/ml.
Reactivity
Species independent
Immunogen
MethA murine fibrosarcoma
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
N-sulfo-rich heparan sulfate
Antigen description
Heparan sulfate (HS) proteoglycans are expressed on cell surfaces and in the extracellular matrix, and take part in developmental, regenerative, as well as pathological processes. By interaction with extracellular matrix components, growth factors, enzymes, and their inhibitors, they regulate and influence tissue distribution of the cells and biological activities of the proteins. N-sulfated heparan sulfate proteoglycans are located in specific microdomains in the plasma membrane, independent on those formed around N-acetyl-rich heparan sulfate, and play different role in the cell signaling.
1P-821_FC_Histogram
Separation of EA.hy926 cells (red-filled) from EA.hy926 cells treated with heparinase (1 hour, 1 UI per ml of cell suspension) stained using anti-N-sulfated heparan sulfate (HepSS-1) PE antibody (concentration in sample 10 μg/ml).

Product specific references:

Kure S, Yoshie O: A syngeneic monoclonal antibody to murine Meth-A sarcoma (HepSS-1) recognizes heparan sulfate glycosaminoglycan (HS-GAG): cell density and transformation dependent alteration in cell surface HS-GAG defined by HepSS-1. J Immunol. 1986 Dec 15;137(12):3900-8.
PubMed
van den Born J, Salmivirta K, Henttinen T, Ostman N, Ishimaru T, Miyaura S, Yoshida K, Salmivirta M: Novel heparan sulfate structures revealed by monoclonal antibodies. J Biol Chem. 2005 May 27;280(21):20516-23.
PubMed
Lipscombe RJ, Nakhoul AM, Sanderson CJ, Coombe DR: Interleukin-5 binds to heparin/heparan sulfate. A model for an interaction with extracellular matrix. J Leukoc Biol. 1998 Mar;63(3):342-50.
PubMed
Crim RL, Audet SA, Feldman SA, Mostowski HS, Beeler JA: Identification of linear heparin-binding peptides derived from human respiratory syncytial virus fusion glycoprotein that inhibit infectivity. J Virol. 2007 Jan;81(1):261-71. Epub 2006 Oct 18.
PubMed
Chung JS, Shiue LH, Duvic M, Pandya A, Cruz PD Jr, Ariizumi K: Sézary syndrome cells overexpress syndecan-4 bearing distinct heparan sulfate moieties that suppress T-cell activation by binding DC-HIL and trapping TGF-beta on the cell surface. Blood. 2011 Mar 24;117(12):3382-90.
PubMed
Kure S, Yoshie O, Aso H: Metastatic potential of murine B16 melanoma correlates with reduced surface heparan sulfate glycosaminoglycan. Jpn J Cancer Res. 1987 Nov;78(11):1238-45.
PubMed
Mii Y, Yamamoto T, Takada R, Mizumoto S, Matsuyama M, Yamada S, Takada S, Taira M: Roles of two types of heparan sulfate clusters in Wnt distribution and signaling in Xenopus. Nat Commun. 2017 Dec 7;8(1):1973.
PubMed
Variant
0.1 mg
1P-821-C100
In stock
264.00 USD
Variant
0.1 mg
1P-821-C100
In stock
264.00 USD

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