Exbio — Research products — Antibodies — CD and related antigens — Anti-Rt PLSCR1 Ascites

Anti-Rt PLSCR1 Ascites

Regulatory status

RUO

Antigen

PLSCR1

Clone

13A6 [TEC-23]

Format

Ascites

Reactivity

Rat

Application

IP, WB, FC (QC tested), ICC

Variant

0.1 ml

21-495-R100

Ask for delivery term HERE

176.00 USD

0.025 ml

21-495-R025

Delivery 1 week

88.00 USD

Variant

0.1 ml

21-495-R100

Ask for delivery term HERE

176.00 USD

0.025 ml

21-495-R025

Delivery 1 week

88.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The antibody 13A6 [TEC-23] binds to an intracellular epitope of rat Phospholipid Scramblase 1 (PLSCR1), an 37-49 kDa protein, accelerating bidirectional movement of plasma membrane phospholipids under conditions of elevated calcium.

Application

IP, WB, FC (QC tested), ICC

Application details

Western blotting: Recommended dilution: 1 μg/ml, positive control: RBL (rat basophilic leukemia) cell line; both reducing and non-reducing conditions.
Flow cytometry: Intracellular staining.

Reactivity

Rat

Immunogen

Pooled lipid raft fraction isolated from RBL-2H3 cells

Formulation

Stabilized with 15mM sodium azide.

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

PL scramblase 1, phospholipid scramblase 1

Antigen description

PLSCR1 (phospholipid scramblase 1) is a multiply palmitoylated endofacial plasma membrane protein containing several SH3 and WW domain binding motives. In the plasma membrane, PLSCR1 plays a role in transbilayer lipid redistributions and signal transduction. Nonpalmitoylated PLSCR1, however, is able to be transported into the nucleus and bind DNA. PLSCR1 potentiates the antiviral activity of interferon and its expression is highly induced by interferons and growth factors.

Entrez Gene ID 117540

UniProt ID P58195

Detection of PLSCR1 in rat basophilic leukemia (RBL) cell line lysate (A) and in PLSCR1 immunoprecipitate from RBL lysate (B). The antibody 13A6 (Tec-23) was used both for immunoprecipitation and immunodetection.

Immunofluorescence staining of PLSCR1 in rat basophilic leukemia (RBL) cell line using 13A6 (Tec-23) antibody.

General references:

Pastorelli C, Veiga J, Charles N, Voignier E, Moussu H, Monteiro RC, Benhamou M: IgE receptor type I-dependent tyrosine phosphorylation of phospholipid scramblase. J Biol Chem. 2001 Jun 8;276(23):20407-12. Epub 2001 Mar 20.
PubMed

Silverman RH, Halloum A, Zhou A, Dong B, Al-Zoghaibi F, Kushner D, Zhou Q, Zhao J, Wiedmer T, Sims PJ: Suppression of ovarian carcinoma cell growth in vivo by the interferon-inducible plasma membrane protein, phospholipid scramblase 1. Cancer Res. 2002 Jan 15;62(2):397-402.
PubMed

Dong B, Zhou Q, Zhao J, Zhou A, Harty RN, Bose S, Banerjee A, Slee R, Guenther J, Williams BR, Wiedmer T, Sims PJ, Silverman RH: Phospholipid scramblase 1 potentiates the antiviral activity of interferon. J Virol. 2004 Sep;78(17):8983-93.
PubMed

Product specific references:

Smrž D, Lebduška P, Dráberová L, Korb J, Dráber P: Engagement of phospholipid scramblase 1 in activated cells: implication for phosphatidylserine externalization and exocytosis. J Biol Chem. 2008 Apr 18;283(16):10904-18.
PubMed