Anti-Hu CD57 PE

Anti-Hu CD57 PE
Regulatory status
RUO
Antigen
CD57
Clone
TB01
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-158-T100
In stock
242.00 USD

25 tests
1P-158-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-158-T100
In stock
242.00 USD

25 tests
1P-158-T025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgM
Specificity
The mouse monoclonal antibody TB01 recognizes CD57, a carbohydrate extracellular antigen present mainly on NK cells, NK T cells, and in neural tissue.
Workshop
HLDA VI: WS Code NK16
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
A pool of neuroblastoma cell lines
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
HNK1, LEU7, 3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc
Antigen description
CD57, also known as HNK1 or Leu7, is a sulphated trisaccharide (3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc) attached to several glycoproteins, including CD56, myelin glycoprotein PO, and neural cell adhesion molecule L1, as well as on glycolipids and chondroitin sulphate proteoglycans in the nervous system. It serves as a NK cell marker and it is expressed on well differentiated prostate cancers and uveal and cutaneous melanoma. CD57+ T cells are implicated as suppressors of T-cell responses.
Entrez Gene ID 27087
UniProt ID Q9P2W7
1P-158_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD57 (TB01) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
1P-158_FC_Dot-plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD57 (TB01) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD8 (MEM-31) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).
1P-158_FC_Dot-plot_2
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD57 (TB01) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD56 (LT56) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1P-158_FC_Histogram
Separation of human CD57 positive NK cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD57 (TB01) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Lim SA, Kim TJ, Lee JE, Sonn CH, Kim K, Kim J, Choi JG, Choi IK, Yun CO, Kim JH, Yee C, Kumar V, Lee KM: Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy. Cancer Res. 2013 Apr 15;73(8):2598-607.
PubMed
Cunha KS, Caruso AC, Faria PA, Silva LE, Fonseca EC, Geller M, de Moura-Neto RS, Lopes VS: Evaluation of Bcl-2, Bcl-x and cleaved caspase-3 in malignant peripheral nerve sheath tumors and neurofibromas. An Acad Bras Cienc. 2013;85(4):1497-511.
PubMed
Phadwal K, Alegre-Abarrategui J, Watson AS, Pike L, Anbalagan S, Hammond EM, Wade-Martins R, McMichael A, Klenerman P, Simon AK: A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells. Autophagy. 2012 Apr;8(4):677-89.
PubMed
Marquardt N, Béziat V, Nyström S, Hengst J, Ivarsson MA, Kekäläinen E, Johansson H, Mjösberg J, Westgren M, Lankisch TO, Wedemeyer H, Ellis EC, Ljunggren HG, Michaëlsson J, Björkström NK: Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells. J Immunol. 2015 Mar 15;194(6):2467-71.
PubMed
Wangerin H, Kristiansen G, Schlomm T, Stephan C, Gunia S, Zimpfer A, Weichert W, Sauter G, Erbersdobler A: CD57 expression in incidental, clinically manifest, and metastatic carcinoma of the prostate. Biomed Res Int. 2014;2014:356427.
PubMed
Fernandez S, French MA, Price P: Immunosenescent CD57+CD4+ T-cells accumulate and contribute to interferon-γ responses in HIV patients responding stably to ART. Dis Markers. 2011;31(6):337-42.
PubMed
Variant
100 tests
1P-158-T100
In stock
242.00 USD

25 tests
1P-158-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-158-T100
In stock
242.00 USD

25 tests
1P-158-T025
Delivery 1 week
121.00 USD

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