Anti-Hu CD56 PE-DyLight® 594

Anti-Hu CD56 PE-DyLight<sup>®</sup> 594
Regulatory status
RUO
Antigen
CD56
Clone
LT56
Format
PE-DyLight® 594
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
T5-789-T100
In stock
275.00 USD
Variant
100 tests
T5-789-T100
In stock
275.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody LT56 recognizes an extracellular epitope of CD56 (NCAM), a transmembrane glycoprotein expressed ubiquitously in the nervous system and found also on T cells and NK cells.
Workshop
HLDA X
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Cell line KG1a
Preparation
Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-DyLight®594 (PE-DyLight®594) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Other names
NCAM1, MSK39
Antigen description
CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.
Entrez Gene ID 4684
UniProt ID P13591
T5-789_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD56 (LT56) PE-DyLight® 594 antibody (10 μl reagent / 100 μl of peripheral whole blood).
T5-789_FC_DOT-PLOT
Flow cytometry multicolor surface staining of human lymphocytes using anti-human CD56 (LT56) PE-DyLight® 594 antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
T5-789_FC_Histogram
Separation of human CD56 positive CD3 negative NK cells (red-filled) from CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD56 (LT56) PE-DyLight® 594 antibody (10 μl reagent / 100 μl of peripheral whole blood).

General references:

McCluggage WG, McKenna M, McBride HA: CD56 is a sensitive and diagnostically useful immunohistochemical marker of ovarian sex cord-stromal tumors. Int J Gynecol Pathol. 2007 Jul;26(3):322-7.
PubMed
Ohishi Y, Kaku T, Oya M, Kobayashi H, Wake N, Tsuneyoshi M: CD56 expression in ovarian granulosa cell tumors, and its diagnostic utility and pitfalls. Gynecol Oncol. 2007 Oct;107(1):30-8.
PubMed
Jakovcevski I, Mo Z, Zecevic N: Down-regulation of the axonal polysialic acid-neural cell adhesion molecule expression coincides with the onset of myelination in the human fetal forebrain. Neuroscience. 2007 Oct 26;149(2):328-37.
PubMed
Lanier LL, Chang C, Azuma M, Ruitenberg JJ, Hemperly JJ, Phillips JH: Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56). J Immunol. 1991 Jun 15;146(12):4421-6.
PubMed

Product specific references:

Munteanu AN, Surcel M, Huică RI, Isvoranu G, Constantin C, Pîrvu IR, Chifiriuc C, Ulmeanu C, Ursaciuc C, Neagu M: Peripheral immune cell markers in children with recurrent respiratory infections in the absence of primary immunodeficiency. Exp Ther Med 2019, June 26, 1693-1700
PubMed
Variant
100 tests
T5-789-T100
In stock
275.00 USD
Variant
100 tests
T5-789-T100
In stock
275.00 USD

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