Anti-Hu CD38 PE-DyLight® 594

Anti-Hu CD38 PE-DyLight<sup>®</sup> 594
Regulatory status
RUO
Antigen
CD38
Clone
HIT2
Format
PE-DyLight® 594
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
T5-366-T100
Ask for delivery term HERE
275.00 USD

25 tests
T5-366-T025
Delivery 1 week
137.50 USD
Variant
100 tests
T5-366-T100
Ask for delivery term HERE
275.00 USD

25 tests
T5-366-T025
Delivery 1 week
137.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody HIT2 reacts with an extracellular epitope of CD38, a 45 kDa type II transmembrane glycoprotein strongly expressed mainly on plasma cells and activated T and B lymphocytes; it is an antigenic marker of lymphoid cells. Its binding is blocked by daratumumab.
Workshop
HLDA III: WS Code T 155
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human thymocytes in foetus
Preparation
Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-DyLight®594 (PE-DyLight®594) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Other names
ADPRC1, cADPr hydrolase 1, T10, NAD(+) nucleosidase, ADP-ribosyl cyclase 1
Antigen description
CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration.
Entrez Gene ID 952
UniProt ID P28907
T5-366_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD38 (HIT2) PE-DyLight® 594 (4 μl reagent / 100 μl of peripheral whole blood).
T5-366 FC Histogram
Separation of human CD38 positive monocytes (red-filled) from human CD38 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD38 (HIT2) PE-DyLight® 594 (4 μl reagent / 100 μl of peripheral whole blood).

General references:

Cakir-Kiefer C, Muller-Steffner H, Oppenheimer N, Schuber F: Kinetic competence of the cADP-ribose-CD38 complex as an intermediate in the CD38/NAD+ glycohydrolase-catalysed reactions: implication for CD38 signalling. Biochem J. 2001 Sep 1;358(Pt 2):399-406.
PubMed
Lund FE, Muller-Steffner H, Romero-Ramirez H, Moreno-García ME, Partida-Sánchez S, Makris M, Oppenheimer NJ, Santos-Argumedo L, Schuber F: CD38 induces apoptosis of a murine pro-B leukemic cell line by a tyrosine kinase-dependent but ADP-ribosyl cyclase- and NAD glycohydrolase-independent mechanism. Int Immunol. 2006 Jul;18(7):1029-42.
PubMed
Partida-Sanchez S, Gasser A, Fliegert R, Siebrands CC, Dammermann W, Shi G, Mousseau BJ, Sumoza-Toledo A, Bhagat H, Walseth TF, Guse AH, Lund FE. Chemotaxis of mouse bone marrow neutrophils and dendritic cells is controlled by adp-ribose, the major product generated by the CD38 enzyme reaction. J Immunol. 2007 Dec 1;179(11):7827-39.
PubMed

Product specific references:

Leukocyte Typing III., McMichael AJ et al (Eds.), Oxford University Press (1987).
Rozková D, Novotná L, Pytlík R, Hochová I, Kozák T, Bartůnková J, Spísek R: Toll-like receptors on B-CLL cells: expression and functional consequences of their stimulation. Int J Cancer. 2010 Mar 1;126(5):1132-43.
PubMed
Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007 Mar 15;109(6):2545-52.
PubMed
Všianská P, Říhová L, Varmužová T, Suská R, Kryukov F, Mikulášová A, Kupská R, Penka M, Pour L, Adam Z, Hájek R: Analysis of B-cell subpopulations in monoclonal gammopathies. Clin Lymphoma Myeloma Leuk. 2015 Apr;15(4):e61-71.
PubMed
Ratuszny D, Skripuletz T, Wegner F, Groß M, Falk C, Jacobs R, Ruschulte H, Stangel M, Sühs KW: Case report: Daratumumab in a patient with severe refractory anti-NMDA receptor encephalitis. Front Neurol. 2020 Dec 22;11:602102.
PubMed
Variant
100 tests
T5-366-T100
Ask for delivery term HERE
275.00 USD

25 tests
T5-366-T025
Delivery 1 week
137.50 USD
Variant
100 tests
T5-366-T100
Ask for delivery term HERE
275.00 USD

25 tests
T5-366-T025
Delivery 1 week
137.50 USD

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