Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD38 FITC

Anti-Hu CD38 FITC

Regulatory status

RUO

Antigen

CD38

Clone

Format

FITC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1F-366-T100

In stock

198.00 USD

25 tests

1F-366-T025

In stock

99.00 USD

Variant

100 tests

1F-366-T100

In stock

198.00 USD

25 tests

1F-366-T025

In stock

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody HIT2 reacts with an extracellular epitope of CD38, a 45 kDa type II transmembrane glycoprotein strongly expressed mainly on plasma cells and activated T and B lymphocytes; it is an antigenic marker of lymphoid cells. Its binding is blocked by daratumumab.

Workshop

HLDA III: WS Code T 155

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Human thymocytes in foetus

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ADPRC1, cADPr hydrolase 1, T10, NAD(+) nucleosidase, ADP-ribosyl cyclase 1

Antigen description

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration.

Entrez Gene ID 952

UniProt ID P28907

Anti-human CD38 FITC antibody (clone HIT2) works in flow cytometry application.

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats.
Mouse monoclonal anti-human CD38 FITC antibody (clone HIT2) was used in concentration 0.5 µg/ml in stained blood sample (2 x 10⁶ cells).

Staining pattern of Anti-human CD38 FITC antibody (clone HIT2) in dot-plot fluorescence visualization (B cell gate)

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats.
Mouse monoclonal anti-human CD38 FITC antibody (clone HIT2) in concentration 0.5 µg/ml in stained stained blood sample (2 x 10⁶ cells) and Mouse monoclonal anti-human CD27 Pacific Blue™ antibody (clone LT27) was used in concentration 2 µg/ml, respectively.

Staining pattern of Anti-human CD38 FITC antibody (clone HIT2) in dot-plot fluorescence visualization (B cell gate)

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats.
Mouse monoclonal anti-human CD38 FITC antibody (clone HIT2) in concentration 0.5 µg/ml in stained stained blood sample (2 x 10⁶ cells) and Mouse monoclonal anti-human CD138 APC antibody (clone MI15) in concentration 2 µg/ml, respectively.

Reactivity of Anti-human CD38 FITC antibody (clone HIT2) on human peripheral leukocytes

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats.
Suspension of blood leukocytes (2 x 10⁶ cells) was added to the mixture of CD38 FITC antibody (clone HIT2, 0.5 µg/ml in stained blood sample), backbone antibody conjugates and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min. Stained sample was fixed with 2 ml of 10× diluted EXCELLYSE Easy solution (#ED7066) for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

General references:

Cakir-Kiefer C, Muller-Steffner H, Oppenheimer N, Schuber F: Kinetic competence of the cADP-ribose-CD38 complex as an intermediate in the CD38/NAD+ glycohydrolase-catalysed reactions: implication for CD38 signalling. Biochem J. 2001 Sep 1;358(Pt 2):399-406.
PubMed

Lund FE, Muller-Steffner H, Romero-Ramirez H, Moreno-García ME, Partida-Sánchez S, Makris M, Oppenheimer NJ, Santos-Argumedo L, Schuber F: CD38 induces apoptosis of a murine pro-B leukemic cell line by a tyrosine kinase-dependent but ADP-ribosyl cyclase- and NAD glycohydrolase-independent mechanism. Int Immunol. 2006 Jul;18(7):1029-42.
PubMed

Partida-Sanchez S, Gasser A, Fliegert R, Siebrands CC, Dammermann W, Shi G, Mousseau BJ, Sumoza-Toledo A, Bhagat H, Walseth TF, Guse AH, Lund FE. Chemotaxis of mouse bone marrow neutrophils and dendritic cells is controlled by adp-ribose, the major product generated by the CD38 enzyme reaction. J Immunol. 2007 Dec 1;179(11):7827-39.
PubMed

Product specific references:

Leukocyte Typing III., McMichael AJ et al (Eds.), Oxford University Press (1987).

Rozková D, Novotná L, Pytlík R, Hochová I, Kozák T, Bartůnková J, Spísek R: Toll-like receptors on B-CLL cells: expression and functional consequences of their stimulation. Int J Cancer. 2010 Mar 1;126(5):1132-43.
PubMed

Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007 Mar 15;109(6):2545-52.
PubMed

Všianská P, Říhová L, Varmužová T, Suská R, Kryukov F, Mikulášová A, Kupská R, Penka M, Pour L, Adam Z, Hájek R: Analysis of B-cell subpopulations in monoclonal gammopathies. Clin Lymphoma Myeloma Leuk. 2015 Apr;15(4):e61-71.
PubMed

Ratuszny D, Skripuletz T, Wegner F, Groß M, Falk C, Jacobs R, Ruschulte H, Stangel M, Sühs KW: Case report: Daratumumab in a patient with severe refractory anti-NMDA receptor encephalitis. Front Neurol. 2020 Dec 22;11:602102.
PubMed