Anti-Hu CD324 PE

Anti-Hu CD324 PE
Regulatory status
RUO
Antigen
CD324
Clone
67A4
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-588-T100
In stock
242.00 USD

25 tests
1P-588-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-588-T100
In stock
242.00 USD

25 tests
1P-588-T025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 67A4 recognizes an extracellular epitope of CD324 / E-cadherin, an approximately 100 kDa epithelial cell adhesion molecule, whose detection is important for determination of invasive potential of epithelial neoplasms.
Workshop
HLDA VIII
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
T-47D cells
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Cadherin-1, E-cadherin, Uvomorulin, CAM 120/80, UVO, CDHE, ECAD, LCAM, BCDS1, Arc-1
Antigen description
CD324 / E-cadherin is an epithelial cell surface molecule, which provides calcium-dependent homophilic interactions with E-cadherin of another cell. These intaractions take part in morphogenetic programs controlling the maintenance of the structural and functional integrity of epithelia and affect invasive potential of epithelial neoplasms. CD324 / E-cadherin is implicated in cell growth and differentiation, cell recognition, and sorting during developmental morphogenesis, as well as in aggregation-dependent cell survival. CD324 / E-cadherin-mediated cell adhesion system is highly regulated from inside the cell by a number of intracellular signaling pathways.
Entrez Gene ID 999
UniProt ID P12830
1P-588 FC
Flow cytometry analysis (surface staining) of HT-29 (red) / SP2 (blue) cells with anti-CD324 (67A4) PE.

General references:

Pece S, Gutkind JS: Signaling from E-cadherins to the MAPK pathway by the recruitment and activation of epidermal growth factor receptors upon cell-cell contact formation. J Biol Chem. 2000 Dec 29;275(52):41227-33.
PubMed
Pece S, Chiariello M, Murga C, Gutkind JS: Activation of the protein kinase Akt/PKB by the formation of E-cadherin-mediated cell-cell junctions. Evidence for the association of phosphatidylinositol 3-kinase with the E-cadherin adhesion complex. J Biol Chem. 1999 Jul 2;274(27):19347-51.
PubMed
Takeichi M: Cadherin cell adhesion receptors as a morphogenetic regulator. Science. 1991 Mar 22;251(5000):1451-5.
PubMed

Product specific references:

Servet-Delprat C, Vidalain PO, Bausinger H, Manié S, Le Deist F, Azocar O, Hanau D, Fischer A, Rabourdin-Combe C: Measles virus induces abnormal differentiation of CD40 ligand-activated human dendritic cells. J Immunol. 2000 Feb 15;164(4):1753-60.
PubMed
Furio L, Guezennec A, Ducarre B, Guesnet J, Peguet-Navarro J: Differential effects of allergens and irritants on early differentiating monocyte-derived dendritic cells. Eur J Dermatol. 2008 Mar-Apr;18(2):141-7.
PubMed
Kutlesa S, Wessels JT, Speiser A, Steiert I, Müller CA, Klein G: E-cadherin-mediated interactions of thymic epithelial cells with CD103+ thymocytes lead to enhanced thymocyte cell proliferation. J Cell Sci. 2002 Dec 1;115(Pt 23):4505-15.
PubMed
Caberg JH, Hubert PM, Begon DY, Herfs MF, Roncarati PJ, Boniver JJ, Delvenne PO: Silencing of E7 oncogene restores functional E-cadherin expression in human papillomavirus 16-transformed keratinocytes. Carcinogenesis. 2008 Jul;29(7):1441-7.
PubMed
Lin JC, Liao SK, Lee EH, Hung MS, Sayion Y, Chen HC, Kang CC, Huang LS, Cherng JM. Molecular events associated with epithelial to mesenchymal transition of nasopharyngeal carcinoma cells in the absence of Epstein-Barr virus genome. J Biomed Sci. 2009 Nov 24;16:105.
PubMed
Furio L, Guezennec A, Ducarre B, Guesnet J, Peguet-Navarro J: Differential effects of allergens and irritants on early differentiating monocyte-derived dendritic cells. Eur J Dermatol. 2008 Mar-Apr;18(2):141-7.
PubMed
Armeanu S, Bühring HJ, Reuss-Borst M, Müller CA, Klein G: E-cadherin is functionally involved in the maturation of the erythroid lineage. J Cell Biol. 1995 Oct;131(1):243-9.
PubMed
Bühring HJ, Müller T, Herbst R, Cole S, Rappold I, Schuller W, Zhu X, Fritzsch U, Faul C, Armeanu S, Ullrich A, Klein G, Schmidt H: The adhesion molecule E-cadherin and a surface antigen recognized by the antibody 9C4 are selectively expressed on erythroid cells of defined maturational stages. Leukemia. 1996 Jan;10(1):106-16.
PubMed
Novak N, Kraft S, Haberstok J, Geiger E, Allam P, Bieber T: A reducing microenvironment leads to the generation of FcepsilonRIhigh inflammatory dendritic epidermal cells (IDEC). J Invest Dermatol. 2002 Oct;119(4):842-9.
PubMed
Servet-Delprat C, Vidalain PO, Bausinger H, Manié S, Le Deist F, Azocar O, Hanau D, Fischer A, Rabourdin-Combe C: Measles virus induces abnormal differentiation of CD40 ligand-activated human dendritic cells. J Immunol. 2000 Feb 15;164(4):1753-60.
PubMed
Robertson H, Ali S, McDonnell BJ, Burt AD, Kirby JA: Chronic renal allograft dysfunction: the role of T cell-mediated tubular epithelial to mesenchymal cell transition. J Am Soc Nephrol. 2004 Feb;15(2):390-7.
PubMed
Novak N, Kraft S, Haberstok J, Geiger E, Allam P, Bieber T: A reducing microenvironment leads to the generation of FcepsilonRIhigh inflammatory dendritic epidermal cells (IDEC). J Invest Dermatol. 2002 Oct;119(4):842-9.
PubMed
Variant
100 tests
1P-588-T100
In stock
242.00 USD

25 tests
1P-588-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-588-T100
In stock
242.00 USD

25 tests
1P-588-T025
Delivery 1 week
121.00 USD

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