Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD307d PE

Anti-Hu CD307d PE

Regulatory status

RUO

Antigen

CD307d

Clone

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-857-T100

Ask for delivery term HERE

242.00 USD

Variant

100 tests

1P-857-T100

Ask for delivery term HERE

242.00 USD

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Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a kappa

Specificity

The mouse monoclonal antibody A1 recognizes an epitope within extracellular domain of CD307d, a transmembrane glycoprotein expressed mainly on memory B cells.

Workshop

HLDA IX

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

DNA-immunization followed by a boost with CD307d-transfected cells

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

FcRL4, FCRH4, IRTA1

Antigen description

CD307d is a type I transmembrane glycoprotein of the Fc receptor family. It contains two ITIM motifs and one ITSM motif in its cytoplasmic domain. CD307d is expressed mainly on the surface of memory B cells in mucosa-associated lymphoid tissues. It binds to aggregated immunoglobulin molecules (IgA, IgG). Defects of CD307d may play a role in HIV-induced memory B cell dysfunction.

Entrez Gene ID 83417

UniProt ID Q96PJ5

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD307d (A1) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Nagata S, Ise T, Pastan I: Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells. J Immunol. 2009 Jun 15;182(12):7518-26
PubMed

Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7.
PubMed