Anti-Hu CD271 APC-Cy™7

Anti-Hu CD271 APC-Cy™7
Regulatory status
RUO
Antigen
CD271
Clone
NGFR5
Format
APC-Cy™7
Reactivity
Human, Rabbit, Cat, Non-human primates, Ferret
Application
Excitation laser
red (633 nm)
Variant
100 tests
T4-642-T100
Ask for delivery term HERE
275.00 USD
Variant
100 tests
T4-642-T100
Ask for delivery term HERE
275.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody NGFR5 (originally C34C) recognizes an epitope within ammino acids 1 - 160 of CD271/NGFR, a 75 kDa transmembrane glycoprotein of the TNFR superfamily.
Workshop
HCDM listed
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
Reactivity
Human, Rabbit, Cat, Non-human primates, Ferret
Negative species
Mouse, Rat
Immunogen
Purified CD271 protein isolated from human melanoma cell line A875
Preparation
Purified antibody is conjugated with activated tandem dye of allophycocyanin-cyanine 7 (APC-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
Other names
TNFRSF16, NGFR, p75NTR, p75NGFR
Antigen description
CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells.
Entrez Gene ID 4804
UniProt ID P08138
T4-642_FC_Histogram
Separation of SK-MEL-30 cells stained using anti-human CD271 (NGFR5) APC-Cy7 antibody (4 μl reagent per million cells in 100 μl of cell suspension, red-filled) from SK-MEL-30 cells stained using mouse IgG1 isotype control (MOPC-21) APC-Cy7 antibody (concentration in sample 5 μg/ml, same as CD271 APC-Cy7 concentration, black-dashed) in flow cytometry analysis (intracellular staining) of SK-MEL-30 cell suspension.

Product specific references:

Thompson SJ, Schatteman GC, Gown AM, Bothwell M: A monoclonal antibody against nerve growth factor receptor. Immunohistochemical analysis of normal and neoplastic human tissue. Am J Clin Pathol. 1989 Oct;92(4):415-23.
PubMed
Marano N, Dietzschold B, Earley JJ Jr, Schatteman G, Thompson S, Grob P, Ross AH, Bothwell M, Atkinson BF, Koprowski H: Purification and amino terminal sequencing of human melanoma nerve growth factor receptor. J Neurochem. 1987 Jan;48(1):225-32.
PubMed
Schatteman GC, Gibbs L, Lanahan AA, Claude P, Bothwell M: Expression of NGF receptor in the developing and adult primate central nervous system. J Neurosci. 1988 Mar;8(3):860-73.
PubMed
Schatteman GC, Gibbs L, Lanahan AA, Claude P, Bothwell M: Expression of NGF receptor in the developing and adult primate central nervous system. J Neurosci. 1988 Mar;8(3):860-73.
PubMed
Alpers CE, Hudkins KL, Ferguson M, Johnson RJ, Schatteman GC, Bothwell M: Nerve growth factor receptor expression in fetal, mature, and diseased human kidneys. Lab Invest. 1993 Dec;69(6):703-13.
PubMed
Variant
100 tests
T4-642-T100
Ask for delivery term HERE
275.00 USD
Variant
100 tests
T4-642-T100
Ask for delivery term HERE
275.00 USD

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