Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD184 PE-Cy™5

Anti-Hu CD184 PE-Cy™5

Regulatory status

RUO

Antigen

CD184

Clone

12G5

Format

PE-Cy™5

Reactivity

Human, Non-human primates

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

T8-146-T100

Ask for delivery term HERE

242.00 USD

Variant

100 tests

T8-146-T100

Ask for delivery term HERE

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a kappa

Specificity

The mouse monoclonal antibody 12G5 recognizes an extracellular epitope of CD184, a 45 kDa G-protein-linked CXC chemokine receptor widely expressed on blood and tissue cells.

Workshop

HLDA VII: WS Code 70204

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human, Non-human primates

Immunogen

CP-MAC-infected Sup-T1 cells

Preparation

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 5 (PE-Cy™5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Cy™ and CyDye™ are registered trademarks of Cytiva.

Other names

CXCR4, LAP3, LCR1, HM89, fusin, SDF1R

Antigen description

CD184, also known as CXCR4 or fusin, is a receptor for the C-X-C chemokine SDF-1. It is expressed mainly in hematopoietic cells, vascular endothelium, and neural tissue. CD184 is a G-protein coupled receptor containing extracellular N-terminal, seven transmembrane domains and intracellular C-terminal domain. It transduces signal by increasing the intracellular calcium level. CD184 plays an essential role in vascularization of the gastrointestinal tract, and is involved in cerebellar development and in hematopoiesis. It is also a coreceptor (with CD4) for HIV-1 X4 virus and a primary receptor for some HIV-2 isolates.

Entrez Gene ID 7852

UniProt ID P61073

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD184 (12G5) PE-Cy™5 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD184 positive lymphocytes (red-filled) from CD184 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD184 (12G5) PE-Cy™5 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Koopman G, Mortier D, Hofman S, Koutsoukos M, Bogers WM, Wahren B, Voss G, Heeney JL: Acute-phase CD4+ T-cell proliferation and CD152 upregulation predict set-point virus replication in vaccinated simian-human immunodeficiency virus strain 89.6p-infected macaques. J Gen Virol. 2009 Apr;90(Pt 4):915-26.
PubMed

Campioni D, Zauli G, Gambetti S, Campo G, Cuneo A, Ferrari R, Secchiero P: In vitro characterization of circulating endothelial progenitor cells isolated from patients with acute coronary syndrome. PLoS One. 2013;8(2):e56377.
PubMed

Uy GL, Rettig MP, Motabi IH, McFarland K, Trinkaus KM, Hladnik LM, Kulkarni S, Abboud CN, Cashen AF, Stockerl-Goldstein KE, Vij R, Westervelt P, DiPersio JF: A phase 1/2 study of chemosensitization with the CXCR4 antagonist plerixafor in relapsed or refractory acute myeloid leukemia. Blood. 2012 Apr 26;119(17):3917-24.
PubMed

Grundler R, Brault L, Gasser C, Bullock AN, Dechow T, Woetzel S, Pogacic V, Villa A, Ehret S, Berridge G, Spoo A, Dierks C, Biondi A, Knapp S, Duyster J, Schwaller J: Dissection of PIM serine/threonine kinases in FLT3-ITD-induced leukemogenesis reveals PIM1 as regulator of CXCL12-CXCR4-mediated homing and migration. J Exp Med. 2009 Aug 31;206(9):1957-70.
PubMed