Anti-Hu CD158d PE-Cy™5

Anti-Hu CD158d PE-Cy™5
Regulatory status
RUO
Antigen
CD158d
Clone
mAb#33
Format
PE-Cy™5
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
T8-609-T100
In stock
242.00 USD
Variant
100 tests
T8-609-T100
In stock
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody mAb#33 (also known as mAb 33 or 33) recognizes extracellular portion of CD158d / KIR2DL4, a 45 kDa NK cell marker. Cell surface expression and function of CD158d / KIR2DL4 depends on genotype of particular individuals.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
NK3.3 cells and KIR2DL4-Ig fusion protein
Preparation
Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 5 (PE-Cy™5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
Other names
KIR2DL4, KIR103AS, 103AS, 15.212
Antigen description
CD158d / KIR2DL4 is a KIR family member that shares structural features with both activating and inhibitory receptors and may mediate different functions under different circumstances. It contains cytoplasmic ITIM, suggesting inhibitory function, but also transmembrane domain similar to those of activating KIRs. It has been reported that CD158d serves as an inhibitory receptor for peripheral and uterine NK cells, but its ligation with soluble mAbs (unlike immobilized mAbs) results in activation of IFN-γ secretion. CD158d also binds both membrane form and soluble form of its ligand HLA-G.
Entrez Gene ID 3805
UniProt ID Q99706
T8-609_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD158d (mAb#33) PE-Cy™5 antibody (4 μl reagent / 100 μl of peripheral whole blood).
T8-609_FC_Dot-plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD158d (mAb#33) PE-Cy™5 antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD56 (LT56) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
T8-609_FC_Histogram
Separation of human CD158d positive CD56 positive NK cells (red-filled) from CD158d negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD158d (mAb#33) PE-Cy™5 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Rajagopalan S, Bryceson YT, Kuppusamy SP, Geraghty DE, van der Meer A, Joosten I, Long EO. Activation of NK cells by an endocytosed receptor for soluble HLA-G. PLoS Biol. 2006 Jan;4(1):e9.
PubMed
Yan WH, Fan LA: Residues Met76 and Gln79 in HLA-G alpha1 domain involve in KIR2DL4 recognition. Cell Res. 2005 Mar;15(3):176-82.
PubMed
LeMaoult J, Zafaranloo K, Le Danff C, Carosella ED: HLA-G up-regulates ILT2, ILT3, ILT4, and KIR2DL4 in antigen presenting cells, NK cells, and T cells. FASEB J. 2005 Apr;19(6):662-4.
PubMed
Rajagopalan S, Fu J, Long EO: Cutting edge: induction of IFN-gamma production but not cytotoxicity by the killer cell Ig-like receptor KIR2DL4 (CD158d) in resting NK cells. J Immunol. 2001 Aug 15;167(4):1877-81.
PubMed
Goodridge JP, Witt CS, Christiansen FT, Warren HS: KIR2DL4 (CD158d) genotype influences expression and function in NK cells. J Immunol. 2003 Aug 15;171(4):1768-74.
PubMed
Variant
100 tests
T8-609-T100
In stock
242.00 USD
Variant
100 tests
T8-609-T100
In stock
242.00 USD

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