Anti-Hu CD123 PerCP-Cy™5.5

Anti-Hu CD123 PerCP-Cy™5.5
Regulatory status
RUO
Antigen
CD123
Clone
6H6
Format
PerCP-Cy™5.5
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
T9-700-T100
In stock
330.00 USD

25 tests
T9-700-T025
Delivery 1 week
165.00 USD
Variant
100 tests
T9-700-T100
In stock
330.00 USD

25 tests
T9-700-T025
Delivery 1 week
165.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 6H6 recognizes an extracellular epitope of CD123 (interleukin 3 receptor alpha), a 60-70 kDa transmembrane protein expressed by myeloid precursors, megakaryocytes, macrophages, dendritic cells, mast cells, basophils, and some B cells. This antibody does not inhibit IL-3 binding to its receptor.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells
Preparation
Purified antibody is conjugated with activated tandem dye of activated Peridinin-Chlorophyll Protein-Cyanine 5.5 (PerCP-Cy™5.5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
Other names
IL3RA, MGC34174, hIL-3Ra
Antigen description
CD123 is the alpha chain of interleukin 3 receptor (IL-3R alpha). This subunit heterodimerizes with the interleukin 3 receptor beta chain (CD131), which is shared with other receptors. CD123 interacts with IL-3 specifically, but with low affinity, and association with the beta subunit confers high affinity binding to the receptor heterodimer. Both chains are required for signaling, but receptor activation and signal transduction depend on IL-3 binding to CD123 as the initial step.
Entrez Gene ID 3563
UniProt ID P26951
T9-700 FC
Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD123 (6H6) PerCP-Cy™5.5.
T9-700_FC_Histogram
Separation of human CD123 positive cells (red-filled) from CD123 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD123 (6H6) PerCP-Cy™5.5 antibody (4 μl reagent / 100 μl of peripheral whole blood).
T9-700_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD123 (6H6) PerCP-Cy™5.5 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Sun Q, Woodcock JM, Rapoport A, Stomski FC, Korpelainen EI, Bagley CJ, Goodall GJ, Smith WB, Gamble JR, Vadas MA, Lopez AF: Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist. Blood. 1996 Jan 1;87(1):83-92.
PubMed
Martín-Gayo E, Sierra-Filardi E, Corbí AL, Toribio ML: Plasmacytoid dendritic cells resident in human thymus drive natural Treg cell development. Blood. 2010 Jul 1;115(26):5366-75.
PubMed
Herling M, Teitell MA, Shen RR, Medeiros LJ, Jones D: TCL1 expression in plasmacytoid dendritic cells (DC2s) and the related CD4+ CD56+ blastic tumors of skin. Blood. 2003 Jun 15;101(12):5007-9.
PubMed
Variant
100 tests
T9-700-T100
In stock
330.00 USD

25 tests
T9-700-T025
Delivery 1 week
165.00 USD
Variant
100 tests
T9-700-T100
In stock
330.00 USD

25 tests
T9-700-T025
Delivery 1 week
165.00 USD

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