Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu C3aR Purified

Anti-Hu C3aR Purified

Regulatory status

RUO

Antigen

C3aR

Clone

HC3aRZ8

Format

Purified

Reactivity

Human

Application

FC (QC tested), IHC(F)

Variant

0.1 mg

11-882-C100

In stock

154.00 USD

Variant

0.1 mg

11-882-C100

In stock

154.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b

Specificity

The mouse monoclonal antibody HC3aRZ8 recognizes an extracellular epitope of C3aR, a transmembrane chemotactic receptor for C3a anaphylatoxin.

Application

FC (QC tested), IHC(F)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

Human C3aR transfectants

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

AZ3B, HNFAG09

Antigen description

C3aR is a 7TM transmembrane protein associated with G proteins, and serves as a receptor for C3a complement fragment. It is expressed mainly on neutrophils, mast cells, basophils, eosinophils, dendritic cells, monocytes, and macrophages. Upon detection of its ligand, the activated C3aR signaling cascade results in degranulation, superoxide production, and chemotaxis.

Entrez Gene ID 719

UniProt ID Q16581

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human C3aR (HC3aRZ8) purified antibody (concentration in sample 1,7 μg/ml, GAM APC).

Separation of human neutrophil granulocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human C3aR (HC3aRZ8) purified antibody (concentration in sample 1,7 μg/ml, GAM APC).

Product specific references:

Abdelbaset-Ismail A, Borkowska-Rzeszotek S, Kubis E, Bujko K, Brzeźniakiewicz-Janus K, Bolkun L, Kloczko J, Moniuszko M, Basak GW, Wiktor-Jedrzejczak W, Ratajczak MZ: Activation of the complement cascade enhances motility of leukemic cells by downregulating expression of HO-1. Leukemia 2017 Feb;31(2):446-458.
PubMed

Larsen LF, Juel-Berg N, Hansen A, Hansen KS, Mills EN, van Ree R, Rådinger M, Poulsen LK, Jensen BM: No difference in human mast cells derived from peanut allergic versus non-allergic subjects. Immun Inflamm Dis. 2018 Dec;6(4):416-427
PubMed

Reis ES, Chen H, Sfyroera G, Monk PN, Köhl J, Ricklin D, Lambris JD: C5a receptor-dependent cell activation by physiological concentrations of desarginated C5a: insights from a novel label-free cellular assay. J Immunol. 2012 Nov 15;189(10):4797-805.
PubMed

Soruri A, Kiafard Z, Dettmer C, Riggert J, Köhl J, Zwirner J: IL-4 down-regulates anaphylatoxin receptors in monocytes and dendritic cells and impairs anaphylatoxin-induced migration in vivo. J Immunol. 2003 Mar 15;170(6):3306-14.
PubMed

Kiafard Z, Tschernig T, Schweyer S, Bley A, Neumann D, Zwirner J: Use of monoclonal antibodies to assess expression of anaphylatoxin receptors in tubular epithelial cells of human, murine and rat kidneys. Immunobiology 2007;212(2):129-39.
PubMed